Camptothecin (CPT), a pentacyclic monoterpene indole alkaloid, is a potent anticancer molecule. A simple and reproducible UV-visible spectrophotometric method for the quantitative determination of Camptothecin (CPT) from Nothapodytes nimmoniana was developed and validated as per ICH guidelines. The parameters linearity, precision, accuracy, limit of detection, limit of quantitation were studied. The concentration of CPT present in stem bark of Nothapodytes nimmoniana was found to be 1.654±0.04 % w/w. Recovery studies were carried out by standard addition method. Recovery of camptothecin at all the level was found to be in the range of 98.76%-99.88%. The developed method was found to be accurate, simple, precise and rapid and can effectively applied for routine analysis.
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